Extracting extremophilic lipases from aqueous streams by using biocompatible ionic liquids

In this work, biocompatible ionic liquids based on aminoacids were employed as extractants to separate extremolipases from aqueous streams. First, the influence of aminoacid and dipeptide-based (cholinium glycinate, ChGly, cholinium glycylglycinate, ChGlygly) lipolytic activity a commercial lipase Candida antarctica (CaLB) in-house synthesized extremophilic lipases Thermus thermophilus HB27 (TtHB27L) Halomonas sp. LM1C (HL) was investigated. The combination thermophilic enzyme with ChGly turned out be optimum for maximizing biocatalytic performance, clearly improving levels attained when water exclusively solvent also surpassing provided CaLB. salting capacity in solutions biodegradable surfactants Tergitol 15S7 15S9 discussed, recording immiscibility areas almost covering all ternary diagrams. biphasic systems experimentally characterized by determining both tie-lines solubility curves at several temperatures data modelled relevant equations like Merchuk, Othmer-Tobias Bancroft ones, they are most common ones describe kind equilibrium data. So, applied extract 313.15 K, achieving very high extraction (about 100 %) TtHB27L, which surpasses maximum values observed 80 %). Finally, process simulated real scale through SuperPro Designer v.8.5 production 385 Kg/year extremolipase.